FIG. 2.

Correlation between 6-h cytokine assay and 7-day ⁵¹Cr release assay in 12 donors. The statistical analysis was performed using GraphPad (San Diego, Calif.) software, assuming that the data are sampled from Gaussian populations with a two-tailed distribution. (A) Frequencies of CD8⁺ IFN-γ⁺ T lymphocytes following 6 h of activation with HLA A2 peptide on day 0 correlated with percent specific lysis of target cells in a ⁵¹Cr release assay performed after 7 days of in vitro stimulation of PBMC with HLA A2-restricted peptide. (B) Frequencies of CD8⁺ IFN-γ⁺ T cells obtained in the day 7 CFC assay (6-h restimulation) correlated with percent specific lysis of target cells in a ⁵¹Cr-release assay also done on day 7.