Table 3.

Variance analysis of WBY protein extraction rate.

Source of Variance	Sum of Squares	Free Degree	Mean Square Deviation	F Value	p Value	Significance
Model	1591.21	9	176.80	21.67	0.0003	**
X1	183.36	1	183.36	22.47	0.0021	**
X2	198.01	1	198.01	24.26	0.0017	**
X3	105.85	1	105.85	12.97	0.0087	**
X1X2	69.72	1	69.72	8.54	0.0222	*
X1X3	68.89	1	68.89	8.44	0.0228	*
X2X3	12.60	1	12.60	1.54	0.2540	N
X12	304.57	1	304.57	37.32	0.0005	**
X22	354.83	1	354.83	43.48	0.0003	**
X32	194.98	1	194.98	23.89	0.0018	**
Residual	57.12	7	8.16
Lack of Fit	19.23	3	6.41	0.6767	0.6103	N
Pure Error	37.89	4	9.47
Cor Total	1648.34	16
R2	0.9653

**-Very significant difference (p < 0.01); *-Significant difference (p < 0.05); N-Insignificant difference (p > 0.05).