Figure 1.

Knockdown of hDHODH affects IAV replication. (A) Expression of hDHODH in A549 cells transfected with hDHODH or scrambled negative control siRNAs. At 48 h after of transfection, total protein extracts were prepared, fractionated by 10% SDS-PAGE, and analyzed by immunoblotting with anti-hDHODH mAbs. Immunodetection of GAPDH was used as a control for protein loading. Molecular weight markers are shown beside the left side of each panel. (B) VRA was performed in A549 cells mock-transfected or transfected with hDHODH or scrambled siRNAs. At 24 h after transfection, cells were infected with IAV, and after 48 h p.i., the cell supernatants were harvested and titrated by the plaque assay in MDCK cells. The data shown are the means ± SDs of two independent experiments performed in triplicate and analyzed by the unpaired t-test, corrected at FDR q < 0.05. ** (p < 0.001) compared to the calibrator sample (Mock).