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. 2022 Oct 17;14(10):2281. doi: 10.3390/v14102281

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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MEDS433 impairs the expression of IAV HA and NA proteins. MDCK cells were infected with IAV at an MOI of 0.1 PFU/cell and, where indicated, treated with 0.5 μΜ MEDS433 or DMSO as a control. Total cell protein extracts were prepared at the indicated times p.i., fractionated by 8% SDS-PAGE, and analyzed by immunoblotting with anti-IAV HA and anti-IAV NA pAbs. Vinculin immunodetection was used as a control for protein loading. Molecular weight markers are shown at the left side of each panel.