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. 2022 Oct 17;14(10):2281. doi: 10.3390/v14102281

Figure 5.

Figure 5

Uridine and orotic acid reversed the inhibitory effect of MEDS433 on IAV replication. (A,B) PRAs were performed in IAV-infected MDCK cells treated before, during, and post-infection with 0.4 μΜ MEDS433 in the presence of increasing concentrations of uridine or cytidine (A) and dihydroorotic acid or orotic acid (B). At 48 h p.i., the viral plaques were stained and microscopically counted. (C,D) VRAs were performed in A549 cells infected with IAV and treated with 0.4 μΜ MEDS433 in the presence of increasing concentrations of uridine (C) and dihydroorotic acid or orotic acid (D). At 48 h p.i., the cell supernatants were harvested and titrated in MDCK cells. Data shown represent means ± SDs of three independent experiments performed in triplicate and analyzed by a one-way ANOVA, followed by Dunnett’s multiple comparison test. * (p < 0.01); ** (p < 0.001); *** (p < 0.0001) compared to the calibrator sample (MEDS433 alone).