Figure 6.

Effects of the combination of MEDS433 and dipyridamole on IAV replication. VRAs were performed in A549 cells treated with MEDS433 alone or in combination with different concentrations of DPY. At 48 h p.i., the cell supernatants were harvested and titrated by the plaque assay in MDCK cells. The effect of the combination was then analyzed by the CompuSyn software [36] and displayed as a fractional effect analysis (Fa) plot in relation to the compound concentrations. The anti-IAV activity of MEDS433 and DPY when used as single agents is depicted by red and green Fa curves, respectively. The effect of the MEDS433-DPY combination is shown with black squares. Results are representative of three independent experiments performed in triplicate and analyzed by a two-way ANOVA, followed by Dunnett’s multiple comparison test. Statistical analysis was performed by comparing MEDS433-treated samples with the MEDS433 + DPY-treated samples for each condition. * (p < 0.01); *** (p < 0.0001).