Figure 7.

A combination of modulators of pyrimidine metabolism inhibits IAV replication in the presence of exogenous uridine. VRAs were performed in A549 cells treated with MEDS433 alone or in combination with different concentrations of DPY. Where indicated, infected cells were supplemented with 20 μM uridine. At 48 h p.i., the cell supernatants were harvested and titrated by the plaque assay in MDCK cells. The data shown represent means ± SDs of three independent experiments performed in triplicate, and MEDS433-treated samples were analyzed by an unpaired t-test, corrected at FDR q < 0.05. * (p < 0.01); ** (p < 0.001) compared to the calibrator sample (MEDS433 + 20 μM uridine, green column).