Figure 8.

The combination of MEDS433 with NHC is synergistic against IAV replication. A549 cells were treated with different concentrations of MEDS433 alone or in combination with various concentrations of NHC before and during IAV infection. At 48 h p.i., the cell supernatants were harvested and titrated for IAV by the plaque assay in MDCK cells. Viral titers were analyzed by the CompuSyn software 1.0 [36], and the anti-IAV activity is shown as a fractional effect analysis (Fa) plot in relation to the drugs’ concentrations. Red and blue Fa curves represent the activity of MEDS433 or NHC on IAV replication when employed as single agents, respectively. The effect of the MEDS433–NHC combination is shown with black triangles. Results are representative of three independent experiments performed in triplicate.