Table 2.

Analysis of the effects of the combination of MEDS433 and DPY against IAV replication.

MEDS433 Concentration (Fold of EC50 a) + DPY 3 μM	MEDS433/DPY CI b	Drug Combination Effect c of MEDS433 and DPY
4×	0.824 ± 0.023	Moderate Synergism
2×	0.607 ± 0.116	Synergism
1×	0.341 ± 0.025	Synergism
0.5×	0.203 ± 0.044	Strong Synergism
0.25×	0.126 ± 0.032	Strong Synergism

^a The EC₅₀ values of MEDS433 were determined by VRAs in IAV-infected A549 cells, as described in the Materials and Methods. ^b Combination index (CI), obtained by computational analysis with the CompuSyn software 1.0. Reported values represent means ± SDs of data derived from n = 3 independent experiments in triplicate. ^c According to the method of Chou [31], drug combination effects are defined as: strong synergism for 0.1 < CI < 0.3; synergism for 0.3 < CI < 0.7; moderate synergism for 0.7 < CI < 0.85; slight synergism for 0.85 < CI < 0.90; nearly additive for 0.90 < CI < 1.10; slight antagonism for 1.10 < CI < 1.20; moderate antagonism for 1.20 < CI < 1.45.