Fig. 2.
Validation of the RBD neutralization test by comparison with the conventional and pseudovirus neutralization assays. (A) Plot showing the activity of SARS-CoV-2 RBD-specific neutralizing antibodies in sera obtained by sVNT against serum titers that yield 50% virus neutralization (IC50) using the cVNT test (26 samples). (B) Plot showing the activity of SARS-CoV-2 RBD-specific neutralizing antibodies in sera obtained by sVNT against sera titers at which pseudovirus neutralization of 50% (IC50) was achieved (pVNT test) (29 samples). r2 is the coefficient of determination. In all the serum samples where no anti-RBD neutralizing antibodies were detected (25 samples), neutralization of the SARS-CoV-2 infection was not detected in all the tests