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IBC induced apoptosis in human breast cancer MDA-MB-231 cells. (A) Flow cytometric analysis of cell death after being treated at different concentrations of IBC using annexin V-FITC/PI dual staining. (B) The cells were treated with IBC for 24 h, subjected to DAPI, PI, and FITC staining and visualized using fluorescence microscopy. (C) Western blotting analysis of Bax, Bcl-2, Akt, and p-Akt-473 protein levels.
