Figure 6.

Protective effects of different concentrations of IDE, the IDE/HP-β-CD inclusion complex, non-overloaded CS NPs, and the overloaded CS NPs-PC on U373 cells against H₂O₂ damage. Cells were treated with all samples for 24 h. After that, they were incubated with H₂O₂ (700 μM) for 1 h. LDH release was assessed as described in Section 3. Results are presented as the means of three different experiments (six replicates for each point) ± standard deviation. No significant reduction in LDH release has been recorded from cells treated with all realized IDE-loaded formulations compared to the treated cells with free IDE.