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. 2022 Oct 27;17(10):e0277051. doi: 10.1371/journal.pone.0277051

Fig 1. Experimental plan.

Fig 1

A. Schematic showing the CRISPR-Cas9 engineering of all three genotypes at codon 200 (E/E–isotype control, E/K heterozygous mutation, K/K homozygous mutation). B. Diagram of the experimental plan to grow the organoids until they contain mostly mature cells (around 4 months old) and add to their media HNE or MGx (green/purple dots) or HSV1 (pink virus) and monitor for the emergence of misfolded species (brown deposits). Created with Biorender. C. Representative RT-QuIC fluorescence traces (showing the mean and standard deviation of 4 replicate reactions per test condition) from sCJD MM1-infected E200K-129MM organoids as compared with MM1 sCJD-infected 129MM and MV2 sCJD-infected 129MV control organoids at >150 dpi.