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. 2022 Oct 27;17(10):e0277051. doi: 10.1371/journal.pone.0277051

Fig 2. HNE and MGx oxidative insults do not cause PrP misfolding or accumulation of seeding activity after short- and longer-term exposure.

Fig 2

A. Western blots of the E200K donor lines one week following exposure to HNE and MGx (3F4 primary antibody). Shown are protease (PK) digested PrP as compared with sCJD PK digested brain tissue (upper) and undigested PrP compared with undigested sCJD brain homogenate (lower) from the E200K donor E/E, E/K and K/K genotype organoids. Numbers above lanes indicate individual organoids, *denotes band likely to be due to incomplete digestion. B & C. RT-QuIC assays for all genotypes and both treatments at 1 week (B) of treatment and after (C) 3 months of weekly treatments. Traces shown are averages of 4 replicate reactions per ‘n’ individual organoids, with the ‘n’ indicated on each graph. NKD = no-known disease donor. None of the samples exceeded the 25% positive well threshold to be considered positive. Control reactions are shown in S4 Fig.