Figure 3.

rs2270363 regulates NMRAL1 expression. (A) CRISPR-Cas9-mediated deletion of the 9-bp sequence containing rs2270363. (B) Deletion of rs2270363 (9 bp) led to upregulation of NMRAL1. However, CORO7 expression was not altered (C). (D and E) MAX knockdown altered NMRAL1 expression in neuronal cells (SH-SY5Y). (F and G) USF1 knockdown affected NMRAL1 expression in neuronal cells. Unpaired two-tailed Student’s t-test was used to determine if the difference reached significance level. n = 3. Data are presented as mean ± SD. ns = not significant. (H and I) Expression of NMRAL1 and CORO7 showed significant downregulation in the brains of schizophrenia cases compared with controls (http://www.szdb.org/).⁵⁰ Schizophrenia cases, n = 258; controls, n = 279.