TABLE 1.
Culture conditionsc | 4 days after restimulationb
|
7 days after restimulationb
|
||||
---|---|---|---|---|---|---|
Total no. of cells (106) | % of CD4+ cells | No. of CD4+ cells (105) | Total no. of cells (106) | % of CD4+ cells | No. of CD4+ cells (105) | |
Medium | 1.2 | 14.87 | 1.78 | 1.1 | 38.88 | 4.28 |
NCC2461 (107 CFU/ml) | 1.2 | 11.88 | 1.43 | 1.0 | 19.9 | 1.99 |
NCC2461 (106 CFU/ml) | 1.2 | 8.94 | 1.07 | 1.1 | 24.09 | 2.65 |
LPS (1 μg/ml) | 1.5 | 12.26 | 1.84 | 1.2 | 21.81 | 2.62 |
aIL-4 (10 μg/ml) | 1.5 | 11.03 | 1.65 | 0.9 | 31.84 | 2.87 |
Values are mean of triplicate cultures from one representative experiment. The SEM (not shown) did not exceed 12% of the means.
CD4+ T cells were harvested, and viability was assessed by trypan blue exclusion 4 and 7 days after the second weekly restimulation.
Culture conditions during the first 2 weeks of culture.