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. 2001 Jul;8(4):695–701. doi: 10.1128/CDLI.8.4.695-701.2001

TABLE 1.

CD4+ T-cell viability after 2 weekly stimulations of MLR cocultured with L. paracasei NCC2461a

Culture conditionsc 4 days after restimulationb
7 days after restimulationb
Total no. of cells (106) % of CD4+ cells No. of CD4+ cells (105) Total no. of cells (106) % of CD4+ cells No. of CD4+ cells (105)
Medium 1.2 14.87 1.78 1.1 38.88 4.28
NCC2461 (107 CFU/ml) 1.2 11.88 1.43 1.0 19.9 1.99
NCC2461 (106 CFU/ml) 1.2 8.94 1.07 1.1 24.09 2.65
LPS (1 μg/ml) 1.5 12.26 1.84 1.2 21.81 2.62
aIL-4 (10 μg/ml) 1.5 11.03 1.65 0.9 31.84 2.87
a

Values are mean of triplicate cultures from one representative experiment. The SEM (not shown) did not exceed 12% of the means. 

b

CD4+ T cells were harvested, and viability was assessed by trypan blue exclusion 4 and 7 days after the second weekly restimulation. 

c

Culture conditions during the first 2 weeks of culture.