| Antibody |
Anti-chicken IgY Alexa Fluor 568-conjungated (Goat polyclonal) |
Abcam |
175477 |
1:500 |
| Antibody |
Anti-Digoxigenin-AP, Fab-Fragmente (Sheep polyclonal) |
Sigma-Aldrich |
Roche-11093274910, RRID:AB_2734716
|
1:5000 |
| Antibody |
Anti-GFP antibody (Chicken polyclonal) |
Aves Lab |
#GFP-1020, RRID:AB_10000240
|
1:1000 |
| Antibody |
Anti-phospho-Smad2/Smad3 (Rabbit monoclonal) |
Cell Signaling Technology |
8828, RRID:AB_2631089
|
1:5000 |
| Antibody |
Anti-phospho-Smad1/Smad5/Smad9 (Rabbit monoclonal) |
Cell Signaling Technologies |
13820 S, RRID:AB_2493181
|
1:100 |
| Antibody |
Anti-rabbit Alexa647 IgG (Goat polyclonal) |
Invitrogen |
A21245, RRID:AB_141775
|
1:100 |
| Antibody |
Anti-rabbit horseradish peroxidase (Goat polyclonal) |
Jackson ImmunoResearch |
111-035-003, RRID:AB_2313567
|
1:500 |
| Chemical compound, drug |
DAPI |
Life Technologies |
D1306 |
1:5000 |
| Chemical compound, drug |
FBS |
Biochrom |
S0415 |
|
| Chemical compound, drug |
Nodal inhibitor SB-505124 |
Sigma |
S4696-5MG |
10 µM |
| Chemical compound, drug |
NucleoZol |
Macherey-Nagel |
740404.200 |
|
| Chemical compound, drug |
Pronase |
Roche |
11459643001 |
|
| Commercial assay, kit |
5′/3′ RACE Kit, 2nd Generation |
Roche |
03353621001 |
|
| Commercial assay, kit |
pCR-bluntII TOPO kit |
Thermo Fisher Scientific |
450245 |
|
| Commercial assay, kit |
Platinum SYBR Green qPCR SuperMix-UDG |
Invitrogen |
1173304 |
|
| Commercial assay, kit |
RNeasy MinElute Cleanup kit |
Qiagen |
74204 |
|
| Commercial assay, kit |
RNeasy Mini kit |
Qiagen |
74104 |
|
| Commercial assay, kit |
SP6 mMessage |
Thermo Fisher Scientific |
AM1340 |
|
| Commercial assay, kit |
SuperScript III Reverse Transcriptase |
Invitrogen |
18080044 |
|
| Commercial assay, kit |
TSA Plus Fluorescein Kit |
Perkin Elmer |
NEL741001KT |
TSA plus cyanine 3 |
| Commercial assay, kit |
Wizard SV Gel and PCR Clean-Up System |
Promega |
A9282 |
|
| Gene (Danio rerio) |
acvr1b-a
|
zfin.org
|
ZDB-GENE-980526–527 |
|
| Gene (Danio rerio) |
acvr1b-b
|
zfin.org
|
ZDB-GENE-131120–9 |
|
| Gene (Danio rerio) |
acvr1c
|
zfin.org
|
ZDB-GENE-181207–1 |
|
| Gene (Danio rerio) |
acvr2a-a
|
zfin.org
|
ZDB-GENE-980526–227 |
|
| Gene (Danio rerio) |
acvr2a-b
|
zfin.org
|
ZDB-GENE-120215–23 |
|
| Gene (Danio rerio) |
acvr2b-a
|
zfin.org
|
ZDB-GENE-980526–549 |
|
| Gene (Danio rerio) |
acvr2b-b
|
zfin.org
|
ZDB-GENE-170621–5 |
|
| Other |
Alexa Fluor 647 dextran |
Invitrogen |
D22914 |
See Materials and methods, Microinjections and embryo dechorionation
|
| Other |
Low-melting point agarose |
Lonza |
50080 |
Mounting of samples for lightsheet microscopy |
| Other |
Human ACVR1B |
Uniprot |
P36896 |
Protein sequence |
| Other |
Human ACVR1C |
Uniprot |
Q8NER5 |
Protein sequence |
| Other |
Human ACVR2A |
Uniprot |
P27037 |
Protein sequence |
| Other |
Human ACVR2B |
Uniprot |
Q13705 |
Protein sequence |
| Other |
Mouse ACVR1B |
Uniprot |
Q61271 |
Protein sequence |
| Other |
Mouse ACVR1C |
Uniprot |
Q8K348 |
Protein sequence |
| Other |
Mouse ACVR2A |
Uniprot |
P27038 |
Protein sequence |
| Other |
Mouse ACVR2B |
Uniprot |
P27040 |
Protein sequence |
| Other |
Zebrafish Acvr1b-a |
Uniprot |
P79689 |
Protein sequence |
| Other |
Zebrafish Acvr1b-b |
Uniprot |
F1QZF0 |
Protein sequence |
| Other |
Zebrafish Acvr1c |
Uniprot |
E7F0I3 |
Protein sequence |
| Other |
Zebrafish Acvr2a-a |
Uniprot |
F1QG01 |
Protein sequence |
| Other |
Zebrafish Acvr2a-b |
Uniprot |
A0A0R4ISZ9 |
Protein sequence |
| Other |
Zebrafish Acvr2b-a |
Uniprot |
Q9YGU4 |
Protein sequence |
| Other |
Zebrafish Acvr2b-b |
Uniprot |
A0A0G2KN81 |
Protein sequence |
| Peptide, recombinant protein |
BamHI |
NEB |
R3136 |
|
| Peptide, recombinant protein |
Alt-R S.p. Cas9 Nuclease V3 |
IDT |
1081058 |
|
| Peptide, recombinant protein |
ClaI-HF |
NEB |
R0197 |
|
| Peptide, recombinant protein |
EcoRI-HF |
NEB |
R3101 |
|
| Peptide, recombinant protein |
Exonuclease I (ExoI) |
NEB |
M0568 |
|
| Peptide, recombinant protein |
NotI-HF |
NEB |
R3189 |
|
| Peptide, recombinant protein |
Recombinant Shrimp Alkaline Phospatase (rSAP) |
NEB |
M0371L |
|
| Peptide, recombinant protein |
StuI (Eco147I) |
Thermo Fisher |
FD0424 |
|
| Peptide, recombinant protein |
T7 endonuclease I (T7E1) |
NEB |
M0302 |
|
| Peptide, recombinant protein |
XbaI |
NEB |
R0145 |
|
| Peptide, recombinant protein |
XhoI |
NEB |
R0146 |
|
| Recombinant DNA reagent |
Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promoters |
Hwang et al., 2013
|
MLM3613 RRID:Addgene_42251
|
|
| Recombinant DNA reagent |
pCS2+-acvr1b-a (plasmid) |
Generated in this study |
|
See Materials and methods, mRNA synthesis
|
| Recombinant DNA reagent |
pCS2+-acvr1b-b (plasmid) |
Generated in this study |
|
See Materials and methods, mRNA synthesis
|
| Recombinant DNA reagent |
pCS2+-acvr1c (plasmid) |
Generated in this study |
|
See Materials and methods, mRNA synthesis
|
| Recombinant DNA reagent |
pCS2+-acvr2a-a (plasmid) |
Generated in this study |
|
See Materials and methods, mRNA synthesis
|
| Recombinant DNA reagent |
pCS2+-acvr2a-b (plasmid) |
Generated in this study |
|
See Materials and methods, mRNA synthesis
|
| Recombinant DNA reagent |
pCS2+-acvr2b-a (plasmid) |
Generated in this study |
|
See Materials and methods, mRNA synthesis
|
| Recombinant DNA reagent |
pCS2+-acvr2b-b (plasmid) |
Generated in this study |
|
See Materials and methods, mRNA synthesis
|
| Recombinant DNA reagent |
TOPO-acvr1b-a (plasmid) |
Generated in this study |
|
See Materials and methods, Whole-mount in situ hybridization
|
| Recombinant DNA reagent |
TOPO-acvr1b-b (plasmid) |
Generated in this study |
|
See Materials and methods, Whole-mount in situ hybridization
|
| Recombinant DNA reagent |
TOPO-acvr1c (plasmid) |
Generated in this study |
|
See Materials and methods, Whole-mount in situ hybridization
|
| Recombinant DNA reagent |
TOPO-acvr2a-a (plasmid) |
Generated in this study |
|
See Materials and methods, Whole-mount in situ hybridization
|
| Recombinant DNA reagent |
TOPO-acvr2a-b (plasmid) |
Generated in this study |
|
See Materials and methods, Whole-mount in situ hybridization
|
| Recombinant DNA reagent |
TOPO-acvr2b-a (plasmid) |
Generated in this study |
|
See Materials and methods, Whole-mount in situ hybridization
|
| Recombinant DNA reagent |
TOPO-acvr2b-b (plasmid) |
Generated in this study |
|
See Materials and methods, Whole-mount in situ hybridization
|
| Sequence-based reagent |
Morpholino antisense oligo control |
Gene Tools, LLC |
|
|
| Sequence-based reagent |
Gene-specific crRNA |
IDT |
|
|
| Sequence-based reagent |
Alt-R CRISPR-Cas9 tracrRNA |
IDT |
1072532 |
|
| Software, algorithm |
Blast |
Uniprot |
RRID:SCR_002380
|
|
| Software, algorithm |
CFX Manager Software |
Bio-Rad |
1845000, RRID:SCR_017251
|
|
| Software, algorithm |
CHOPCHOP |
Montague et al., 2014
|
RRID:SCR_015723
|
|
| Software, algorithm |
Clustal Omega |
Madeira et al., 2019
|
RRID:SCR_001591
|
|
| Software, algorithm |
Excel |
Microsoft |
RRID:SCR_016137
|
|
| Software, algorithm |
Fiji |
Schindelin et al., 2012
|
RRID:SCR_002285
|
|
| Software, algorithm |
Hetindel, Version 1 |
https://github.com/najasplus/hetindel_shinyapp
|
RRID:SCR_018922
|
|
| Software, algorithm |
Jalview version 2.10.3b1 |
Waterhouse et al., 2009
|
RRID:SCR_006459
|
|
| Software, algorithm |
Lasergene Seqman Pro 14 |
DNASTAR |
|
|
| Software, algorithm |
Matlab |
Mathworks |
http://mathworks.com RRID: SCR_001622
|
|
| Software, algorithm |
PolyPeakParser |
Hill et al., 2014
|
|
|
| Software, algorithm |
PyFDAP |
Bläßle and Müller, 2015
|
RRID:SCR_022664
|
|
| Software, algorithm |
PyFRAP |
Bläßle et al., 2018
|
RRID:SCR_022665
|
|
| Software, algorithm |
R |
R-Core-Team |
RRID:SCR_001905
|
|
| Strain, stain background (E. coli) |
DH5α |
In-house |
|
Chemically competent |
| Strain, strain background (Danio rerio) |
acvr1b-at03pm zebrafish mutant |
Generated in this study |
|
See Materials and methods, Fish lines and husbandry
|
| Strain, strain background (Danio rerio) |
acvr1ct06pm zebrafish mutant |
Generated in this study |
|
See Materials and methods, Fish lines and husbandry
|
| Strain, strain background (Danio rerio) |
acvr2a-asa34654 zebrafish mutant |
EZRC |
sa34654 |
|
| Strain, strain background (Danio rerio) |
acvr2a-bsa18285 zebrafish mutant |
EZRC |
sa18285 |
|
| Strain, strain background (Danio rerio) |
acvr2b-at08pm zebrafish mutant |
Generated in this study |
|
See Materials and methods, Fish lines and husbandry
|
| Strain, strain background (Danio rerio) |
H2A.F/Z:GFP |
Pauls et al., 2001
|
|
|
| Strain, strain background (Danio rerio) |
MZsqt-/-;cyc-/-
|
Feldman et al., 1998; Schier et al., 1996; Ciruna et al., 2002
|
|
Mutants were obtained by germ line transplantation |
| Strain, strain background (Danio rerio) |
oeptz57 zebrafish mutant |
Gritsman et al., 1999; Zhang et al., 1998
|
tz57 |
|
| Strain, strain background (Danio rerio) |
TE zebrafish |
In-house |
|
Wild type |
