Table 1.
Parameter | Characteristic | Healthy-LB (n = 21) | PCOS-LB (n = 22) | p value | FDR | Healthy-HB (n = 20) | PCOS-HB (n = 25) | p value | FDR | Four groups p value | Four groups FDR |
---|---|---|---|---|---|---|---|---|---|---|---|
Demographic characteristics | Age (years) | 28.53 ± 3.19 | 28.96 ± 3.87 | 0.6 | 0.832 | 29.9 ± 2.98 | 29.04 ± 3.89 | 0.283 | 0.498 | 0.555 | 0.666 |
BMI (kg/m2) | 20.01 ± 1.83 | 20.68 ± 2.17 | 0.23 | 0.504 | 29.03 ± 3.06 | 28.19 ± 2.82 | 0.332 | 0.541 | 0.001 | 0.001 | |
Liver function | LDH (U/L) | 174.2 ± 29.51 | 174.96 ± 44.35 | 0.942 | 0.972 | 183.8 ± 30.79 | 198.88 ± 30.71 | 0.121 | 0.272 | 0.013 | 0.025 |
AST (U/L) | 20.2 ± 6.09 | 20.1 ± 5.73 | 0.808 | 0.972 | 19.62 ± 4.9 | 24.88 ± 10.51 | 0.071 | 0.209 | 0.121 | 0.166 | |
ALT (U/L) | 14.77 ± 7.95 | 18.19 ± 11.92 | 0.526 | 0.827 | 19.96 ± 10.31 | 32.35 ± 26.24 | 0.043 | 0.144 | 0.002 | 0.004 | |
GGT (U/L) | 20 ± 9.69 | 20.91 ± 9.58 | 0.551 | 0.832 | 21.97 ± 9.72 | 28.92 ± 14.32 | 0.044 | 0.144 | 0.021 | 0.039 | |
ALP (U/L) | 69.67 ± 16.8 | 69 ± 16.12 | 0.444 | 0.748 | 76.29 ± 16.11 | 78.04 ± 23.73 | 0.592 | 0.723 | 0.114 | 0.162 | |
CHE (U/mL) | 7.32 ± 1.49 | 7.96 ± 1.35 | 0.294 | 0.579 | 9.02 ± 1.32 | 9.6 ± 1.54 | 0.371 | 0.583 | 0.001 | 0.001 | |
MAO (U/L) | 3.29 ± 0.91 | 3 ± 1.12 | 0.303 | 0.579 | 5.42 ± 2.74 | 4.1 ± 1.47 | 0.128 | 0.272 | 0.001 | 0.001 | |
AFU (U/L) | 23.34 ± 4.34 | 27.23 ± 6.57 | 0.043 | 0.142 | 26.95 ± 5.69 | 29.32 ± 6.5 | 0.326 | 0.541 | 0.031 | 0.056 | |
TP (g/L) | 76.28 ± 4.51 | 77.01 ± 3.86 | 0.972 | 0.972 | 76.77 ± 4.77 | 76.56 ± 3.83 | 0.955 | 1 | 0.998 | 0.998 | |
ALB (g/L) | 44.1 ± 2.12 | 44.79 ± 1.94 | 0.338 | 0.619 | 45.69 ± 6.92 | 44.74 ± 2.14 | 0.53 | 0.707 | 0.676 | 0.782 | |
GLB (g/L) | 32.19 ± 3.03 | 32.22 ± 2.89 | 0.674 | 0.899 | 32.41 ± 3.92 | 31.82 ± 2.79 | 0.576 | 0.723 | 0.952 | 0.987 | |
ALB/GLB | 1.38 ± 0.11 | 1.4 ± 0.12 | 0.601 | 0.832 | 1.39 ± 0.18 | 1.41 ± 0.15 | 0.479 | 0.68 | 0.822 | 0.928 | |
TBIL (μmol/L) | 10.87 ± 4.56 | 10.94 ± 4.37 | 0.971 | 0.972 | 11.84 ± 5.65 | 10.45 ± 2.93 | 0.743 | 0.861 | 0.932 | 0.987 | |
DBIL (μmol/L) | 2.05 ± 0.94 | 1.96 ± 0.81 | 0.933 | 0.972 | 2.51 ± 2.4 | 1.77 ± 0.57 | 0.15 | 0.3 | 0.56 | 0.666 | |
IBIL (μmol/L) | 8.82 ± 3.7 | 8.99 ± 3.67 | 0.846 | 0.972 | 9.33 ± 3.75 | 8.67 ± 2.46 | 1 | 1 | 0.964 | 0.987 | |
Renal function | CO2 (mmol/L) | 25.17 ± 2.48 | 26.22 ± 2.19 | 0.178 | 0.421 | 25.42 ± 7.61 | 25.62 ± 2.56 | 0.098 | 0.239 | 0.099 | 0.15 |
BUN (mmol/L) | 4.8 ± 1.28 | 4.09 ± 0.71 | 0.032 | 0.12 | 4.74 ± 0.94 | 4.59 ± 1.14 | 0.418 | 0.634 | 0.067 | 0.114 | |
Cr (μmol/L) | 75.62 ± 7.22 | 71.19 ± 8.67 | 0.103 | 0.282 | 72.5 ± 11.08 | 71.68 ± 9.5 | 0.561 | 0.723 | 0.105 | 0.153 | |
Metabolic index | UA (μmol/L) | 346.39 ± 89.7 | 355.47 ± 74.5 | 0.605 | 0.832 | 404.96 ± 138.48 | 428.22 ± 79.55 | 0.234 | 0.428 | 0.005 | 0.009 |
TC (mmol/L) | 4.74 ± 0.83 | 4.74 ± 0.61 | 0.913 | 0.972 | 4.84 ± 0.72 | 4.8 ± 0.87 | 0.508 | 0.699 | 0.884 | 0.972 | |
TG (mmol/L) | 0.97 ± 0.39 | 1.06 ± 0.45 | 0.452 | 0.748 | 1.37 ± 0.65 | 1.43 ± 0.78 | 0.991 | 1 | 0.049 | 0.085 | |
HDL-C (mmol/L) | 1.58 ± 0.22 | 1.46 ± 0.29 | 0.096 | 0.282 | 1.1 ± 0.18 | 1.16 ± 0.16 | 0.226 | 0.428 | 0.001 | 0.001 | |
LDL-C (mmol/L) | 2.89 ± 0.66 | 2.94 ± 0.42 | 0.762 | 0.972 | 3.18 ± 0.58 | 3.15 ± 0.67 | 0.811 | 0.91 | 0.24 | 0.301 | |
Sex hormone | LH (mIU/mL) | 6.12 ± 1.65 | 12.23 ± 7.73 | 0.004 | 0.027 | 3.17 ± 1.69 | 6.89 ± 3.29 | 0.001 | 0.001 | 0.001 | 0.001 |
FSH (mIU/mL) | 4.58 ± 2.11 | 6.6 ± 2.04 | 0.001 | 0.015 | 5.64 ± 1.59 | 6.27 ± 1.68 | 0.085 | 0.225 | 0.002 | 0.003 | |
LH/FSH | 2.05 ± 3.15 | 1.93 ± 1.16 | 0.156 | 0.402 | 0.56 ± 0.2 | 1.23 ± 0.95 | 0.001 | 0.002 | 0.001 | 0.001 | |
PRL (ng/mL) | 26.15 ± 16.61 | 15.84 ± 7.79 | 0.015 | 0.064 | 20.5 ± 13.46 | 18.15 ± 10.28 | 0.624 | 0.742 | 0.092 | 0.146 | |
E2 (pg/mL) | 28.58 ± 18.32 | 69.08 ± 50.55 | 0.001 | 0.001 | 32.97 ± 14.52 | 44.13 ± 15.74 | 0.033 | 0.144 | 0.001 | 0.001 | |
PROG (nmol/L) | 0.9 ± 0.3 | 0.64 ± 0.37 | 0.008 | 0.039 | 0.64 ± 0.44 | 0.54 ± 0.48 | 0.087 | 0.225 | 0.001 | 0.003 | |
Androgen | AD (ng/mL) | 1.2 ± 0.37 | 1.99 ± 0.9 | 0.004 | 0.027 | 0.87 ± 0.32 | 1.8 ± 0.65 | 0.001 | 0.001 | 0.001 | 0.001 |
TT (ng/mL) | 0.31 ± 0.13 | 0.51 ± 0.28 | 0.033 | 0.12 | 0.19 ± 0.08 | 0.46 ± 0.23 | 0.001 | 0.001 | 0.001 | 0.001 | |
DHEA (ng/mL) | 10.11 ± 5.27 | 11.02 ± 8.88 | 0.953 | 0.972 | 5.57 ± 4.41 | 9.72 ± 4.71 | 0.005 | 0.031 | 0.004 | 0.008 | |
FAI | 1.83 ± 0.99 | 5.18 ± 3.35 | 0.001 | 0.002 | 2.32 ± 1.27 | 8.78 ± 4.93 | 0.001 | 0.001 | 0.001 | 0.001 | |
SHBG (nmol/L) | 68.1 ± 31.72 | 40.3 ± 17.5 | 0.002 | 0.022 | 32.91 ± 13.34 | 21.75 ± 11.81 | 0.005 | 0.031 | 0.001 | 0.001 | |
Glucose tolerance | G0 (mmol/L) | 4.77 ± 0.38 | 5.16 ± 0.42 | 0.005 | 0.027 | 5.19 ± 0.69 | 5.67 ± 1.34 | 0.479 | 0.68 | 0.001 | 0.001 |
G120 (mmol/L) | 5.76 ± 1.33 | 7.41 ± 2.44 | 0.007 | 0.039 | 6.98 ± 1.68 | 9.18 ± 4.09 | 0.023 | 0.123 | 0.001 | 0.001 | |
Insulin | I0 (mIU/L) | 8.33 ± 3.65 | 12.36 ± 14.26 | 0.459 | 0.748 | 18.63 ± 9.21 | 18.52 ± 10.68 | 0.848 | 0.91 | 0.001 | 0.001 |
I120 (mIU/L) | 52.3 ± 34.95 | 92.41 ± 78.14 | 0.046 | 0.142 | 106.17 ± 85.51 | 134.32 ± 71.02 | 0.13 | 0.272 | 0.001 | 0.002 | |
HOMA-IR | 1.8 ± 0.87 | 2.94 ± 3.73 | 0.269 | 0.564 | 4.27 ± 2.13 | 4.67 ± 2.7 | 0.847 | 0.91 | 0.001 | 0.001 | |
Methylation | FKBP5-Met1 | 51.86 ± 3.4 | 50.61 ± 2.41 | 0.182 | 0.421 | 54.59 ± 6.13 | 51.24 ± 4.78 | 0.041 | 0.144 | 0.093 | 0.146 |
FKBP5-Met2 | 80.41 ± 4.41 | 80.51 ± 4.07 | 0.857 | 0.972 | 84.81 ± 8.54 | 79.72 ± 7.3 | 0.046 | 0.144 | 0.161 | 0.208 | |
FKBP5-Met | 66.14 ± 3.64 | 65.56 ± 2.67 | 0.839 | 0.972 | 69.7 ± 7.06 | 65.48 ± 5.79 | 0.045 | 0.144 | 0.153 | 0.203 |
The data are shown as the mean ± SD
BMI Body mass index, LDH Lactate dehydrogenase, AST Aspartate aminotransferase, ALT Alanine aminotransferase, GGT Glutamyltransferase, ALP Alkaline phosphatase, CHE Cholinesterase, MAO Monoamine oxidase, AFU α-L-fucosidase, TP Total protein, ALB Albumin, GLB Globulin, TBIL Total bilirubin, DBIL Direct bilirubin, IBIL Indirect bilirubin, BUN urea nitrogen, Cr Creatinine, UA Uric acid, TG Triglycerides, TC Total cholesterol, HDL-C High-density lipoprotein cholesterol, LDL-C Low-density lipoprotein cholesterol, LH Luteinizing hormone, FSH Follicle stimulating hormone, PRL Prolactin, E2 Estrogen, PROG Progesterone, AD Androstenedione, TT Total testosterone, DHEA Dehydroepiandrosterone, FAI free androgen index, SHBG Sex hormone-binding globulin, G0 glucose level at fasting status, G120 Glucose level at 120 min after glucose load, I0 insulin level at fasting status, I120 insulin level at 120 min after glucose load, HOMA-IR homeostasis model assessment for IR, FKBP5-Met1 FKBP5 DNA methylation at CpG 35,657,180/hg19, FKBP5-Met2 FKBP5 DNA methylation at CpG 35,657,202/hg19, FKBP5-Met average of FKBP5 DNA methylation at CpG 35,657,180 and 35,657,202/hg19