Extended Data Fig. 4.
a. NF-kB - secreted alkaline phosphatase reporter 24 or 72 hours after treatment with increasing doses of viral-like particles (VLP, made with packaging plasmid and VSV-G but no genome). N = 2 independent experiments. b. Quantification of IL-1β in supernatants from MDM mock infected or infected with HIV-1(M), HIV-2 or HIV-1(O). N = 4 donors. c. IRF-reporter activation after interferon β (IFNβ) or IL-1β treatment of THP-1 cells. N = 4 independent experiments. d. % of infection in THP-1 cells after addition of IFNβ or IL-1β at different time points. Dotted line indicates the % of infection of untreated cells. N = 3 independent experiments e. Infection levels in MDM depleted of TRIM5 with equal genome copies of VSV-G pseudotyped HIV-1(M), HIV-2 or HIV-1(O) –GFP measured 48 h post-infection. N = 2 donors. Data shows mean + SD.