Extended Data Fig. 7.
a, b. Secreted CXCL10 (A) IL8 (B) (ELISA) 48hpi and GAPDH-normalised mRNA levels in MDM (fold induction over uninfected 24hpi). c. % Infection of HIV-1(M) CA Q50Y 120 R in non-targeting CRISPR (NT-KO) or MAVS CRISPR knock out (MAVS-KO) cells. d. GAPDH-normalised mRNA levels, (fold induction over uninfected), in non-targeting CRISPR control (NT-KO) or MAVS KO THP-1 cells 24hpi. e. IRF-luciferase reporter 48 h after infection by equal genome copies of VSV-G-pseudotyped HIV-1(M) WT or Q50Y 120R mutant in ThP1 cells Ctrl or overexpressing TREX. f. Infection measured at 48 hours corresponding to (e) by flow. Mean + /− SD, n = 3 independent experiments or donors. g. VSV-G pseudotype titration curves in U87 cells expressing non-targeting control shRNA (shCtrl) or TRIM5 targeting shRNA (shTRIM5). Mean + /− SD n = 2 independent experiments. a, b two-tailed unpaired t-test vs untreated MDM. *p < 0.05, **p < 0.01. e, f paired t-test vs THP-1 Ctrl vector. ***p < 0.001.