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. 2022 Oct 26;7(11):1762–1776. doi: 10.1038/s41564-022-01247-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1. — a. Infection of MDM with HIV, measured at 48 h by counting Gag positive cells, in the presence of anti-interferon α/β receptor (IFNα/β-R), or control, antibody (cAb). b. Replication of HIV-1(M), HIV-2 or HIV-1(O) isolates in permissive GHOST cells measuring induced GFP expression by flow. Representative experiment of 2 independent replicates. c, d. Activation of (c) IRF-luciferase reporter or (d) NF-kB secreted alkaline phosphatase reporter 48 h after infection by equal genome copies of VSV-G-pseudotyped HIV-1(M), HIV-2 or HIV-1(O) -GFP. e, f. Measurement of VSV-G pseudotyped HIV-1(M), HIV-1(O) and HIV-2 DNA synthesis (GFP primers) during a 20 h time course in THP-1 cells. g. Infection measured at 48 hours in wells parallel to (e) by flow. h. Viral DNA (GFP) copy number at 20 h post-infection per infected cell using data from (e-f). Mean + /− SD, N = 3 donors (MDM) or independent experiments (THP-1 c,d). N = 4 independent experiments ThP1 e-h.

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