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. Author manuscript; available in PMC: 2023 Oct 24.
Published in final edited form as: Curr Biol. 2022 Sep 20;32(20):4438–4450.e5. doi: 10.1016/j.cub.2022.09.007

Figure 7: mAChR-B KD results in unspecific conditioning.

Figure 7:

A. Experimental protocol. Flies were conditioned against MCH using 12 equally spaced 1.25 s electric shocks at 50 V. Flies were then subjected to OCT or MCH for valence evaluation (see methods).

B. MCH or OCT valence (as designated) observed with or without pre-exposure to conditioning against MCH in flies with mAChR-B RNAi 1 driven by R71G10-GAL4 (γ KCs). Following conditioning against MCH both mAChR-B KD and control flies showed the same increase in aversion towards MCH. In contrast, when OCT valence was examined, mAChR-B KD flies showed increased aversion towards OCT whereas the parental controls showed reduced aversion towards OCT (mean ± SEM), n (left to right): MCH: 63, 61,65, 70, 73, 75; OCT: 96, 76, 66, 77, 101, 76; * p < 0.05, ** p<0.01, *** p<0.001; (Kruskal-Wallis Dunn’s correction for multiple comparisons). For detailed statistical analysis see Table S1.

See also Figures S5, S6, and S7, Data S1 and Table S1.