Fig. 4. Mt1-mmp-dependent mouse macrophage-mediated basement membrane remodeling.
a Mt1-mmp immunostaining (green) of mouse macrophages cultured on basement membrane explants (unstained) in control media, polarized with LPS (1 µg/mL) or recombinant mouse IL-4 (20 ng/mL) with relative immunofluorescence quantified. Results are representative of 3 experiments performed with results from a single experiment with Mt1-mmp quantified in 10 randomly selected cells as mean ± SEM with significance determined by two-tailed t test. b Laminin immunofluorescence of basement membranes cultured with LPS-polarized Mt1-mmp+/+ mouse macrophages (MØm) or unstimulated, LPS-, and IL-4-polarized Mt1-mmp−/− mouse cells for 6 days. Results representative of three independent experiments performed. c Quantification of the area of basement membrane degraded as analyzed by ImageJ pixel analysis under each set of conditions from (A). Results are expressed as mean ± SEM (n = 3 independent exps) with significance determined by two-tailed t test. Bars: 10 µm. d Laminin immunofluorescence of Mt1-mmp−/− mouse macrophages transduced with a lentiviral MT1-MMP-mCherry vector (pseudo-colored green) for 48 h before culture on a basement membrane explant (pseudo-colored red) for 6 days. MT1-MMP-mCherry-positive protrusions are localized to basement membrane perforations (arrowheads) in orthogonal cross-sections or viewed en face. Images shown are representative of three independent experiments. Bars: left panels, 10 µm; right panels; 5 µm. Source data are provided as a Source data file.