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. 2022 Oct 27;7:63. doi: 10.1038/s41525-022-00333-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Confocal microscopy (left) and flow cytometry (right) were performed using specific antibodies for CD24 and CD44 labeled with PE and APC, respectively. b, c Suppression of tumorsphere formation activity (b) and invasion activity (c) by treatment with either or both of the anti-CD24 and CD44 antibodies. The tumorsphere assay was conducted for 7 days, and the invasion chamber assay system containing Matrigel was performed for 24 h. The relative number of tumorspheres compared to the percentage of Panc0203 cells group treated by IgG control (b, left). Diameter of tumorspheres (b, right). d Effect of CD24 short hairpin RNA (shRNA) on the invasion activity of Panc0203 and SP1030 cells. An invasion chamber assay system containing Matrigel was performed for 24 h. Data are presented as the mean values ±SD.