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. 2022 Oct 27;13(10):905. doi: 10.1038/s41419-022-05317-3

Fig. 2. Senescent neutrophils secret exosomes to promote the proliferation and chemoresistance of BC cells.

Fig. 2

A Representative confocal microscopy image showing the internalization of PKH67-labeled exosomes (green) by MCF-7 cells. Scare bars, 10 μm. B Cell proliferation as measured by EdU incorporation was promoted by SN-exo in MCF-7 cells. Scare bars, 100 μm. C Migration ability of MCF-7 cells was enhanced by SN-exo, tested by transwell assay. D CCK8 assay of BC cells pre-incubated with indicated exosomes for 48 h followed by docetaxel treatment at indicated concentrations for 48 h. E Colony formation assay of BC cells pre-incubated with indicated exosomes for 48 h followed by docetaxel at indicated concentrations for 48 h. F Quantification of the number of exosomes from senescent neutrophils with indicated treatments. G, H CCK8 assay of BC cells pre-incubated with indicated CM for 48 h followed by docetaxel at different concentrations for 48 h. Data represent the mean ± SD of at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.