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. 2022 Oct 27;13(10):905. doi: 10.1038/s41419-022-05317-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A RNA pulldown was performed with biotinylated ZEB1. Immunoblotting of IGF2BP family and YTH family m6A readers in cell lysate, biotinylated full-length ZEB1 and beads only (NC) in MCF-7^SN-exo cells. B Immunoblotting of ZEB1 full length (#1), ZEB1 3’UTR region (#2), 3’UTR m6A motif mutant ZEB1 (#3), lysate (Ly) and beads only (NC) by RNA pulldown assay in MCF-7^SN-exo and MDA-MB-231^SN-exo cells. C RIP assays showing the direct binding between YTHDF2 protein and ZEB1 mRNA in HCT116 and DLD1 cells. Agarose electrophoresis (up) and qPCR analysis (down). D RIP assays demonstrating the enrichment of YTHDF2 protein bound ZEB1 mRNA in shNC versus shFTO MCF-7^SN-exo and MDA-MB-231^SN-exo cells. E The correlation between YTHDF2 and ZEB1 in breast cancer was analyzed in TCGA. F Expression correlation of YTHDF2 and ZEB1 was analyzed in breast cancer patients (n = 82) using IHC. Scare bars, 100 μm. G Immunoblotting of YTHDF2, FTO and ZEB1 protein levels in MCF-7^SN-exo and MDA-MB-231^SN-exo cells with shNC, FTO knockdown only (shFTO#1), YTHDF2 knockdown only (shYTHDF2) and both FTO and YTHDF2 knockdown (shFTO + shYTHDF2). Data represent the mean ± SD of at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant.