FIG. 4.

Deletion analysis of glycine-rich motifs in UL57/3 (aa 545 to 601). (A) Amino acid sequences of recombinant proteins UL57/3 and UL57/3 mut and the peptide UL57/3-Gly. The glycine-rich motifs are indicated by boxes. Numbers at the top of the sequences indicate amino acids contained in the recombinant proteins and in the peptide. (B) Immunoblot and ELISA reactivity of sera from patients with acute EBV infection (serum samples 2, 3, 8, and 10 correspond to the serum samples in Table 2) or from patients with acute HCMV infection (I to IV). Reactivity in immunoblots and ELISAs is depicted by − (negative), + (moderately reactive), ++ (reactive), +++ (highly reactive), and ++++ (very highly reactive). (C) Coomassie brillant blue-stained polyacrylamide gel and immunoblot of recombinant proteins. Recombinant antigens were subjected to analysis in about equal amounts, as verified by the Coomassie stain. The blot was probed with serum sample II (HCMV infection), providing an example of reactivity. The antigens used are indicated above the gel and the blot, respectively. Positions of molecular weight markers (M) are shown between the gel and blot. SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis.