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. 2022 Oct 27;5:1137. doi: 10.1038/s42003-022-04066-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Body weight: no differences in body weight values found between control and mutant mice. Males: controls n = 6, Bsx cKO n = 3. Females: controls n = 4, Bsx cKO n = 4. Unpaired t-test with Welch’s correction. P > 0.05. b Rotarod: latency to fall from the Rotarod throughout a 3-day test is shown. Males: controls n = 8, Bsx cKO n = 6. Females: controls n = 7, Bsx cKO n = 6. 2-way RM ANOVA. No significant effect of genotype. Gradual improvement was detected for all mice indicating that motor learning has occurred (Effect of Day, 2-way RM ANOVA, Males, P = 0.0003; Females, P < 0.0001). c–j Open field task. c–d distance travelled over a 2-day test. Only mutant males show reduced distance travelled on both days compared to controls. Mann Whitney U test, Day 1, P = 0.025; Day 2, P = 0.014). e, f similar time spent moving and average speed between control and mutant mice over a 2-day test. g diagram showing the open field arena. The centre of the arena is shown as a red box. h similar time spent in centre between female control and mutant mice. i, male mutants showed reduced time in centre on day 2 (Mann Whitney U test, Day 2, P = 0.0215) but this was not significant over a 4-day test period (j). c–i Males: controls n = 13, Bsx cKO n = 9. Females: controls n = 9, Bsx cKO n = 11. j: controls n = 6, Bsx cKO n = 5. k–p Running wheel task: female and male BSX cKO mice show reduced cumulative distance run on the wheel. Females: n = 4 mice for each genotype. 2-way RM ANOVA, Fisher’s LSD test *P < 0.05, **P < 0.01, ***P < 0.001. Distance: effect of Day P < 0.0001. Max speed: effect of Day P < 0.0001. Average speed: effect of Day P < 0.0001. Males: controls n = 11; Bsx cKO n = 9 except day 8 where n = 3 for both groups. Distance: effect of Genotype P < 0.0001, Day P < 0.0001 and Interaction P < 0.0001. Max speed: effect of Genotype P = 0.0004 and Day P < 0.0001. Average speed: effect of Genotype P = 0.0002 and Day P < 0.0001. All graphs show mean ± SEM. Source data are provided in Supplementary Data 1.