Frequency of Spike-specific CD4+T cell using activation-induced markers
(A–E) Frequency (left panel) or fold change (right panel) in respect to w0 of Spike-specific CD4+T cells expressing CD69+CD154+ (A), CD69+CD137+ (B) or CD25+OX40+ (C) in CVID (n = 12, black circles), unPAD (n = 9, black triangles), other PADs (CID: n = 1, open triangles and TID: n = 1, open circles), and HC groups (n = 10, black diamonds) after vaccination. Comparison of Spike-specific CD4+T cell subsets in CVID, unPAD and HC groups at w8 (D), and w28 versus w8 (E). In (D and E), Box-whiskers plots showing Min, Max, median and interquartile range are shown. (A–C) Horizontal dotted line indicates limit of detection and lines indicate median.
Data in (A–C) were analyzed using Wilcoxon signed rank test. Data in (D and E) were analyzed using Dunn’s Multiple Comparison Test. ∗p<0.05; ∗∗p<0.01; ∗∗∗p < 0.001.