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. 2022 Oct 27;12:18116. doi: 10.1038/s41598-022-21425-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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TnC biosensor transient in intact FDB myofibers at 37 °C. (A) Normalized traces (0–100%) of an ensemble average of biosensor ratio fluorescence (blue) and sarcomere length (black) dynamics. Inset shows same data and serves to highlight the region of the peak amplitude (y-axis represents a 50% change from baseline, x-axis represents 0.05 s). (B) Summary statistics for time to peak for the biosensor ratio and sarcomere length shows timing of the peak activation of the biosensor ratio is not significantly different from the time to peak of the sarcomere length (n = 15 myofibers in each group from n = 7 animals). (C) Summary statistics for the time to 50% of baseline for the biosensor ratio and sarcomere length demonstrate that biosensor inactivation (0.0323 ± 0.0036 s) was delayed relative to the relaxation of the myofiber (0.0193 ± 0.0014 s) (n = 15 myofibers in each group from n = 7 animals). (D) Summary statistics for the time to 75% of baseline for the biosensor fluorescence ratio and sarcomere length showed a significant difference in the relaxation dynamics of the biosensor and sarcomere length, with the biosensor inactivation (0.0530 ± 0.0053 s) slower than the sarcomere length relaxation of the myofiber (0.0274 ± 0.0022 s) (n = 15 myofibers in each group from n = 7 animals). Myofibers measured at 37 °C with 0.2 Hz stimulation. Mean ± S.E.M. are presented, *P < 0.05.