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. 2022 Oct 27;12:18116. doi: 10.1038/s41598-022-21425-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Calcium dynamics in isolated intact FDB myofibers at room temperature. (A) Normalized traces (0–100%) of an ensemble average of FURA-2 calcium fluorescence (red) and sarcomere length (black) dynamics showing that the calcium transient time to peak precedes the peak of the sarcomere length transient. Inset shows same data and serves to highlight the differences of the peak timing (y-axis represents a 50% change from baseline, x-axis represents 0.10 s). (B) Summary statistics for time to peak for the calcium transient and sarcomere length changes shows peak amplitude of the calcium transient occurs significantly before the peak sarcomere length change (n = 13 myofibers in each group from n = 3 animals). (C) Summary statistics for the time to 50% of peak for the calcium transient and sarcomere length demonstrates there is a significant difference between the calcium kinetics (0.0037 ± 0.0005 s) and sarcomere length changes (0.0155 ± 0.0018 s) (n = 13 myofibers in each group from n = 3 animals). (D) Summary statistics for the time to 50% of baseline for the calcium transient (0.0508 ± 0.0087 s) and sarcomere length changes (0.0440 ± 0.0056 s) shows there is no significant difference in the recovery dynamics of calcium and sarcomere length relaxation (Sarcomere length: n = 13, FURA-2: n = 12 from n = 3 animals). Myofibers measured at room temperature with 0.2 Hz stimulation. Mean ± S.E.M. are presented, *P < 0.05.