Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 27;12:18116. doi: 10.1038/s41598-022-21425-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Calcium dynamics in isolated intact FDB myofibers at 37 °C. (A) Normalized traces (0–100%) of an ensemble average of FURA-2 calcium fluorescence (red) and sarcomere length (black) dynamics. Inset shows same data and serves to highlight the region of the peak amplitude (y-axis represents a 50% change from baseline, x-axis represents 0.10 s). (B) Summary statistics for time to peak of the calcium transient and sarcomere length changes shows timing of the peak calcium amplitude occurs significantly before the time to peak of the sarcomere length (Sarcomere length: n = 21, FURA n = 19 from n = 2 animals). (C) Summary statistics for the time to 50% of peak for the calcium transient and sarcomere length demonstrates that the calcium transient (0.0038 ± 0.0003 s) is significantly faster than the sarcomere length change (0.0084 ± 0.0006 s) (Sarcomere length: n = 21, FURA n = 19 from n = 2 animals). (D) Summary statistics for the time to 50% of baseline for the FURA-2 calcium transient and sarcomere length changes shows there is a significant difference in the recovery dynamics of the calcium transient and sarcomere length, with the calcium transient (0.0161 ± 0.0015 s) recovering more quickly than the relaxation of the myofiber (0.0228 ± 0.0018 s) (Sarcomere length: n = 21, FURA n = 19 from n = 2 animals). Myofibers measured at 37 °C with 0.2 Hz stimulation. Mean ± S.E.M. are presented, *P < 0.05.