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. 2022 Apr 28;29(11):2177–2189. doi: 10.1038/s41418-022-01007-x

Fig. 5. Downregulation of LRPPRC induced mRNA degradation of MDR1.

Fig. 5

A Pathway enrichment analysis of differentially expressed genes (FC > 1.2, or FC < 0.8, P < 0.05) in Sh-LRPPRC cells (SW480) and NC cells through Reactome gene sets. Fisher’s test was used to calculate the P value for the pathway enrichment analysis. B Correlations of the mRNA expression of LRPPRC and MDR1 in the CCLE database. C Correlations of the mRNA expression of LRPPRC and MDR1 in the TCGA database. D Correlations of the protein expression of LRPPRC and MDR1 in the CPTAC database. E The protein expression of LRPPRC and MDR1 in SW480 cells and SW480R cells with or without LRPPRC knockdown (up). The mRNA expression of LRPPRC and MDR1 in SW480 cells and SW480R cells with or without LRPPRC knockdown (down). F The interaction between MDR1 and LRPPRC was confirmed by RIP and Western blotting. G The mRNA expression of MDR1 in SW480 cells with or without LRPPRC knockdown after treatment with actinomycin D (5 μg/ml). H Immunoblotting of LRPPRC, P53 and MDR1 after transfection with P53 mutation plasmid or negative control in SW480 cells and SW480R cells with or without LRPPRC knockdown. I The IC50 value of 5FU in SW480 cells transfected with P53 mutation plasmid or negative control with or without LRPPRC knockdown. J The protein expression of Bcl2, LRPPRC, and MDR1 in SW480 cells with or without LRPPRC combining treatment with 5FU.