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. 2022 Oct 27;12:18051. doi: 10.1038/s41598-022-21706-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Establishment of a living biobank of CMT organoids. (a) Sampling and generation of organoids from different primary canine epithelial mammary tissues from the same dog: malignant (carcinomas), benign (adenomas), or non-neoplastic mammary tissues. (b) Success rates for establishing 3D in vitro organoids from the indicated mammary tumors and normal mammary epithelium. Values indicate the number of donor tumors from which models were successfully derived versus the total number of donor tumors for a total of 16 dogs. (c) Brightfield images of 3D organoids of mammary tumor (ORG-63-C) and of normal mammary epithelium (ORG-63-N) grown in Basement Membrane Extract 1 day, 7 days (passage 3), and 180 days (passage 13) following isolation. Both organoid lines are derived from dog 63. Scale bar, 50 μm.