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. 2022 Oct 27;12:18051. doi: 10.1038/s41598-022-21706-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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CMT organoids share important morphological characteristics and hormonal status with their primary tissue. (a) Representative images of H&E stainings of primary tissue (first column), patient-derived organoids (second column), and brightfield images (third column) of 3D organoids of normal mammary epithelium (bottom line) and of mammary tumor (top line) grown in Basement Membrane Extract. The green arrows indicate solid organoids, and the orange ones indicate cystic organoids; the blue arrows indicate luminal secretion. For comparison purposes, two different areas of the same scanned slide are represented for the H&E image of ORG-63-N. Scale bar, 50 μm. (b) Histogram showing the distribution of organoids that are hormone receptor-positive (brown) and negative (grey) grouped per original carcinoma receptor status. (c) Representative images of H&E stainings and immunohistochemical analyses of estrogen receptor (ER), human epidermal growth factor receptor 2 (HER2), Ki67 (marker of cell proliferation), cytokeratin (CK) 5/6 (luminal epithelial cells), and p63 (basal cells) in CMT-63-C tumor and tumor-derived organoids ORG-63-C (passage 3). Scale bar, 50 μm. (d) Representative images of H&E stainings and immunohistochemical analyses of estrogen receptor (ER), human epidermal growth factor receptor 2 (HER2), Ki67 (marker of cell proliferation), cytokeratin (CK) 5/6 (luminal epithelial cells), and p63 (basal cells) in normal mammary epithelium (T-63-N) and tissue-derived organoids ORG-63-N (passage 3). Scale bar, 50 μm.