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. 2022 Oct 27;12:18051. doi: 10.1038/s41598-022-21706-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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CMT organoids allow in vitro drug testing. (a) Dose–response curves indicating viability 8 days after treatment with alpelisib for PIK3CA-mutated organoid lines (ORG-51-C4, ORG-MCF7) and wild-type organoid line ORG-51-C3. Error bars represent the standard deviation (SD) of three independent experiments. P-values are calculated by one-way ANOVA followed by Tukey’s multiple comparisons test for the log(IC50) values of the survival curves, *P = 0.0363, **P = 0.0092. As a control, the HBC PIK3CA-mutated, TP53 wild-type MCF-7 cell line was used and grown in BME to form organoids (ORG-MCF7) following the same conditions as the CMT organoids. (b) Dose–response curves indicating viability 8 days after treatment with nutlin-3a for TP53-mutated organoid line ORG-51-C4 and wild-type organoid lines (ORG-51-C3, ORG-MCF7). Error bars represent SD of three independent experiments. P-values are calculated by one-way ANOVA followed by Tukey’s multiple comparisons test for the log(IC50) values of the survival curves, ****P < 0.0001. As a control, the HBC PIK3CA-mutated, TP53 wild-type MCF-7 cell line was used and grown in BME to form organoids (ORG-MCF7) following the same conditions as the CMT organoids.