Table 2.
Primer sequences used for fluorescent quantitation PCR.
| Genes 2 | Genbank | Primer sequences, 5′-3′1 | Size, bp |
|---|---|---|---|
| β-actin | NM_205518.1 | F:ATTGTCCACCGCAAATGCTTC R:AAATAAAGCCATGCCAATCTCGTC |
113 |
| TLR4 | NM_001030693.2 | F:CATCTCTGGAGTTCCTGCTGAA R:TGTATGGATGTGGCACCTTGA |
145 |
| MyD88 | NM_001030962.5 | F:CGGAGGATGGTGGTCGTCATT R:TCGTTCTTCATGGTCTTGCACTTG |
140 |
| NF-κB | NM_001396038.1 | F:CAGCCCATCTATGACAACCG R:TCAGCCCAGAAACGAACCTC |
152 |
| NLRP3 | NM_001348947.2 | F:GAAGGTGCTGCTATGGACATTG R:CGTGCTCTGTGTATTTCTGCTTAT |
118 |
| Bax | XM_422067 | F:TGAGCATGTAGCAACGGAAG R:AGCAAGCTGATTGACGGTCT |
295 |
| Bcl-2 | NM_205339.3 | F:AGGACAACGGAGGATGGGATG R:CACCAGAACCAGGCTCAGGAT |
110 |
1F, Forward primer; R, Reverse primer.
2TLR4, Toll-Like Receptor 4; MyD88, Myeloid Differentiation Primary Response 88; NF-?B, Nuclear Factor Kappa-Light-Chain-Enhancer of Activated B Cells; NLRP3, nod Like Receptor Family Pyrin Domain Containing 3; Bax, BCL2-Associated X; Bcl-2, B-Cell Lymphoma-2.