Fig. 3.

VEGF A gene editing efficiency of Cas9 RNPs in retinal dystrophy in mice. (A) The overall study outline, herein, CNV model was developed in mice using laser followed by subretinal injection of VEGF A targeting Cas9 RNP. After 7 d injection of RPE complexes were analyzed for CNV area and deep sequencing was performed to evaluate gene editing in the targeted cells/tissues. Meanwhile, after 3 d injection of VEGFA ELISA was also performed. (B) Representative laser-induced CNV stained with isolectin B4 (IB4) in C57BL/6 J mice injected with the Rosa26-specific Cas9 RNP (as a control) or the VEGFA-RNP. The area of CNV is demonstrated as yellow line. (C) CNV area. (D) level of VEGF A in the CNV area. (E) Gene editing in terms of indel in RPE cells at VEGFA targeted site. Reprinted from [48], licensed under CC BY 4.0 (https://creativecommons.org/licenses/by/4.0/legalcode). Copyright © 2017 the authors. Published by Cold Spring Harbor Laboratory Press.