Figure 3.

The mutation of Spr2 abolished complex I assembly and activity. A, The BN-PAGE gels were stained with CBB. Crude mitochondrial extracts from endosperm and embryo of spr2-1 and WT immature kernels at 11 DAP. The positions of respiratory complexes I, III, V, and supercomplex I + III₂ are indicated. CI: Complex I, CIII: Complex III, CV: Complex V. B, Detection of NADH dehydrogenase activity of complex I. Dihydrolipoamide dehydrogenase was used as a loading control. C and D, Accumulation of mitochondrial complex III and V in Spr2-1 mutant and WT by western blotting using specific antibodies against Cyt_C1 (C) and ATPase (D). E, Immunodetection analysis with antibodies against Nad9, Cyt_C1, Cox2, ATPase, and AOX.