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. 2022 Aug 16;34(11):4495–4515. doi: 10.1093/plcell/koac253

Figure 4.

Figure 4

WRKY53-OE anthers have lower GA contents and GA biosynthesis gene expression. A, GA contents in developing anthers of LJ11 and WRKY53-OE plants grown under NC. The concentrations and the identity of endogenous GAs were determined using a Thermo Scientific Ultimate 3000 UHPLC. Data are shown as means ± se (n = 3). B, SLR1 protein abundance in panicles of LJ11 and WRKY53-OE plants grown under NC, as detected by immunoblot with an anti-OsSLR1 antibody. ACTIN, detected with an anti-ACTIN antibody, were used as the loading control. C, Relative expression levels of GA biosynthesis genes in developing panicles of LJ11 and WRKY53-OE plants grown under NC. Data are shown as means ± se (n = 4). Each dot represents the result from one biological replicate, error bars indicate means ± se. P-values were calculated by Student’s t test: **P < 0.01, *P < 0.05, and ns indicates no significant difference.