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. 2022 Aug 16;34(11):4623–4640. doi: 10.1093/plcell/koac237

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© The Author(s) 2022. Published by Oxford University Press on behalf of American Society of Plant Biologists.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Complementation of the gsa2 mutant by constitutive expression of p35S:GSA1. A, Comparison of 4-week-old SD-grown seedlings of wild-type (Col-0) gsa1, gsa2 and the two gsa2 lines #1 and #3 expressing 35S:GSA1. B, Chlorophyll a (dark green, bottom) and b (yellow-green, top) contents. C, GSA1 (light green) and GSA2 (dark green) transcript levels. D, Immunoblot analysis of the indicated proteins. RBCL stained with Ponceau red is shown as a loading control. E, GSAAT enzyme activity. F, Relative rates of ALA synthesis in the indicated lines. Leaf material was harvested 4 h after the onset of light from 4-week-old seedlings grown under standard SD conditions. All the data in (B), (E), and (F) are provided as a mean of the standard deviation of biological replicates from three different harvests. Statistical significance compared with Col-0 seedlings was performed by ^aP ≤ 0.05, ^bP ≤ 0.01, ^cP ≤ 0.001 using Student’s t test and is found in Supplemental Data Set S1; fw, fresh weight.