KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| P38 MAPK (D13E1) XP Rabbit mAb#8690 | Cell Signaling | Cat# 8690S; Lot# 9 RRID:AB_10999090 |
| Anti-Cardiac Troponin I antibody | Abcam | Cat# ab56357; Lot# GR313459-8 RRID:AB_880622 |
| phospho-p38 antibody, mouse |
Cell Signaling | Cat#; 9216S Lot# 27 RRID:AB_331296 |
| Hoechst 33342, Trihydrochloride, Trihydrate - 10 mg/mL Solution in Water | Invitrogen | Cat# H3570 Lot#1860907; RRID: N/A |
| Anti-GAPDH antibody produced in rabbit | Sigma | Cat# G9545; Lot# 127M4814V RRID:AB_796208 |
| Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit | Cell Signaling | Cat#: 4370S Lot# 28 RRID:AB_2315112 |
| p44/42 MAPK (Erk1/2) (L34F12) Mouse | Cell Signaling | Cat#; 4696 Lot# 23 RRID:AB_ AB_390780 |
| Anti-Lamin B2 antibody [LN43] | Abcam | Cat# ab8983; Lot# GR141423-12 RRID:AB_306912 |
| NUP Complex Protein Antibody mAb 414 | Abcam | Cat#; ab24609 Lot# GR-3235621-11 RRID:AB_ 448181 |
| Anti-NUP98 mAb | Abcam | Cat#; ab50610 Lot# GR3212026-6[ RRID:AB_ 881769 |
| Anti-NUP107 polyclonal anitbody | Abcam | Cat#; ab73290 Lot# GR6666-28 RRID:AB_1269604 |
| Anti-NUP155 polyclonal anitbody | Novus Biologicals | Cat#; NBP2-19609 Lot# 40429 RRID:AB_2915906 |
| Anti-NUP133 mAb | Santa Cruz | Cat#; sc376763 Lot# E2317 RRID:AB_2889360 |
| c-Jun (60A8) Rabbit mAb | Cell Signaling | Cat#; 9165 Lot# 13 RRID:AB_2130165 |
| c-Jun (60A8) Rabbit mAb (Alexa Fluor 647 conjugate) | Cell Signaling | Cat#; 40502S Lot# 3 RRID:AB_2909794 |
| c-Fos (9F6) Rabbit mAB | Cell Signaling | Cat#; 2250S Lot# 12 RRID:AB_2247211 |
| NF-kB p65 (D14E12) XP Rabbit mAB | Cell Signaling | Cat#; 8242S Lot# 16 RRID: AB_10859369 |
| MYH7-specific Polyclonal Antibody | Proteintech | Cat#; 22280-1-AP Lot# NA RRID: AB_2736821 |
| Myosin Cardiac Heavy Chain Antibody (MYH6) | Genway Biotech | Cat#; GWB-452D0F |
| Lot# NA RRID: AB_10281249 |
||
| Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 | Thermo Fisher | Cat# A21447 Lot# 2045332 RRID: AB_141844 |
| Donkey anti-Mouse IgG (H+L) Highly Cross- Adsorbed Secondary Antibody, Alexa Fluor 488 |
Thermo Fisher | Cat# A21202 Lot# 2018296 RRID: AB_141607 |
| Donkey anti-Mouse IgG (H+L) Highly Cross- Adsorbed Secondary Antibody, Alexa Fluor 568 |
Thermo Fisher | Cat# A10037 Lot# 2110843 RRID: AB_2534013 |
| Donkey anti-Mouse IgG (H+L) Highly Cross- Adsorbed Secondary Antibody, Alexa Fluor 594 |
Thermo Fisher | Cat# A21203 Lot# 1722995 RRID: AB_141633 |
| Donkey anti-Rabbit IgG (H+L) Highly Cross- Adsorbed Secondary Antibody, Alexa Fluor 488 |
Thermo Fisher | Cat# A21206 Lot# 2072687 RRID: AB_2535792 |
| Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 555) | Abcam | Cat# ab150074 Lot# GR3241278-3 RRID: AB_2636997 |
| Bacterial and Virus Strains | ||
| Ad-Dronpa-ERK 1 | This Paper | NA |
| Ad-Lmnb2 | (Han et al., 2020) | NA |
| Chemicals, Peptides, and Recombinant Proteins | ||
| LMNB2 siRNA | GE Dharmacon | Cat# L-005290-00-0005 |
| Control siRNA | GE Dharmacon | NC1269625 |
| NUP 155 siRNA | GE Dharmacon | Cat# E-095619-00-0020 |
| Critical Commercial Assays | ||
| Cellutron Neomyts cardiomyocyte isolation kit | Thermofisher | Cat# NC9073658 |
| Neonatal Cardiomyocyte isolation kit | Milteni Biotec | Cat# 130-100-825 |
| RNeasy Plus micro kit | Qiagen | Cat# 74034 |
| NE-PER™ Extraction Kit | Thermofisher | Cat# 78833 |
| Deposited Data | ||
| RNAseq | GEO repository | GSE186949 |
| Experimental Models: Organisms/Strains | ||
| Mouse: Lmnb2fl/fl: Lmnb2tm1.1Sgy/J | Laboratory of Dr. Stephen Young, UCLA |
JAX stock #032559 |
| Mouse: Tnnt2-Cre: Tg(Tnnt2-cre)5Blh/j | Jackson lab | JAX stock #024240 |
| Mouse: αMHC-cre: Tg(Myh6-cre)2182Mds/j | Jackson lab | JAX stock #011038 |
| Mouse: αMHC-cre; Lmnb2fl/fl | (Han et al., 2020) | N/A |
| Mouse: Tnnt2-cre; Lmnb2fl/fl | (Han et al., 2020) | N/A |
| Oligonucleotides | ||
| Primer: Gapdh
Forward: GTGGACCTGACCTGCCGTCT Reverse: GGAGGAGTGGGTGTCGCTGT |
This paper | N/A |
| Primer: Actb
Forward: GGAGGAGTGGGTGTCGCTGT Reverse: GTCATACTCCTGCTTGCTGAT |
This paper | N/A |
| Primer: Lmnb2
Forward: GAGGACATTGCCTACAAGTTCAC Reverse: TTCCACACAAGGGTTGATG |
This paper | N/A |
| Primer: ACTB
Forward: CCAACCGCGAGAAGATGA Reverse: CCAGAGGCGTACAGGGATAG |
This paper | N/A |
| Primer: Tnnt2 Forward: TTGGCACCCAATGCAGACTCCTGTTT Reverse: CCCGCACTATGCCAGC AATAAA |
This paper | N/A |
| Primer: MYH6- rat
Forward: GCCCTTTGACATCCGCACAGAGT Reverse: TCTGCTGCATCACCTGGTCCTCC |
This paper | N/A |
| Primer: MYH7- rat
Forward: TGGCACCGTGGACTACAATA Reverse: CTACAGGTGCATCAGCTCCA |
This paper | N/A |
| Primer: NUP 155-rat
Forward: GGCAAGTTCAGAGTAAGAGCG Reverse: CGCAGCAGTAAGAGACAGTTC |
This paper | N/A |
| Primer: cJun-rat
Forward: CGACCCCCACTCAGTTCTTGT Reverse: GCAGCGTATTCTGGCTATGCA |
This paper | N/A |
| Primer: cFos- rat
Forward: CCAAGCGGAGACAGATCAACTT Reverse: TCCAGTTTTTCCTTCTCTTTCAGTAGAT |
This paper | N/A |
| Primer: Nppa- rat
Forward: CGTATACAGTGCGGTGTCCAAC Reverse: CATCTTCTCCTCCAGGTGGTCTAG |
This paper | N/A |
| Primer: Nppb- rat
Forward: AAGTCCTAGCCAGTCTCCAGAACA Reverse: TTGAGAGCTGTCTCTGAGCCATT |
This paper | N/A |
| Software and Algorithms | ||
| NIS-Elements | Nikon Instruments | N/A |
| Nikon Element 3D analysis module | CBI Univ. Pittsburgh | N/A |
| Fiji/Image J | NIH | RRID:SCR_002285 https://imagej.nih.gov/ij/ |
| GraphPad Prism 7 | GraphPad Software | N/A |
| NCBI Primer Blast | NCBI | RRID:SCR_004870 |
| Integrated DNA Technologies (IDT) PrimerQuest software | Integrated DNA technologies (IDT) | https://www.idtdna.com › primerquest |
| R | The R Project for Statistical Computing |
https://www.r-project.org/ |