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. 2022 Oct 7;30:311–322. doi: 10.1016/j.omtn.2022.10.001

Figure 1.

Figure 1

MIR145 promoter emerges as potent epigenetic regulator of miR-143/145 cluster in bladder tumors

(A) Schematic representation of MIR143/145 locus, highlighting the CpG sites analyzed in silico within the CpG island of the cluster and the distal, proximal, and core promoters of MIR143 and MIR145 genes. (B) Spearman correlation of miR-143 and miR-145 levels in bladder tumors of the screening (left) and TCGA-BLCA (right) cohorts. (C) Boxplots representing miR-143 and miR-145 levels in bladder tumors and healthy adjacent urothelium in screening (left) and TCGA-BLCA (right) cohorts; p-values calculated by Mann-Whitney U test. (D) Heatmap illustrating the methylation levels of the CpG island and the promoters of MIR143/145 genes based on TCGA-BLCA data; visualized by XENA Browser Visualization Tool. (E–G) Spearman correlation analysis of miR-143/145 expression and methylation levels of CpG island (E), MIR143 (F), and MIR145 (G) promoters. (H, I) Methylation levels of MIR143 (H) and MIR145 (I) proximal and core promoters in bladder tumors and normal adjacent urothelium in TCGA-BLCA cohort visualized by XENA Browser Visualization Tool; p-values calculated by Welch's t-test. ∗p<0.05; ∗∗p<0.01, ∗∗∗p<0.001, ns: not significant.