Table 2.
Amino acid substitutions in the revertants
| 37°C |
38°C |
39°C |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Parent TS strain | Revertantsa | Amino acid substitution | Codon change | Rateb | Revertantsa | Amino acid substitution | Codon change | Rateb | Revertantsa | Amino acid substitution | Codon change | Rateb |
| A50-18 | 19/230 | nsp14 248G | GTG>GGT | 6/19 | 5/223 | nsp14 416G | AGT>GGT | 5/5 | 0/230 | – | – | – |
| nsp14 416G | AGT>GGT | 13/19 | ||||||||||
| H50-11 | 2/230 | nsp16 67V | ATT>GTT | 2/2 | 1/240 | nsp16 67V | ATT>GTT | 1/1 | 0/237 | – | – | – |
| L50-33 | 34/230 | nsp3 445L | TTT>CTT | 4/34 | 39/228 | nsp3 445L | TTT>CTT | 2/39 | 13/230 | nsp3 445L | TTT>CTT | 2/13 |
| TTT>TTG | 17/34 | TTT>TTG | 22/39 | TTT>TTG | 6/13 | |||||||
| TTT>TTA | 1/34 | TTT>TTA | 3/39 | TTT>TTA | 1/13 | |||||||
| nsp3 445C | TTT>TGT | 8/34 | nsp3 445C | TTT>TGT | 7/39 | nsp3 445C | TTT>TGT | 2/13 | ||||
| nsp3 445V | TTT>GTT | 3/34 | nsp3 445V | TTT>GTT | 3/39 | nsp3 445V | TTT>GTT | 2/13 | ||||
| nsp3 445I | TTT>ATT | 1/34 | nsp3 445I | TTT>ATT | 2/39 | |||||||
| L50-40 | 17/230 | nsp3 445L | TTT>CTT | 2/17 | 14/228 | nsp3 445L | TTT>CTT | 2/14 | 4/216 | nsp3 445L | TTT>CTT | 2/4 |
| TTT>TTG | 9/17 | TTT>TTG | 9/14 | TTT>TTG | 1/4 | |||||||
| nsp3 445C | TTT>TGT | 6/17 | nsp3 445C | TTT>TGT | 3/14 | nsp3 445C | TTT>TGT | 1/4 | ||||
a
Number of wells with revertants/Total.
b
Rate of wells containing viruses with the specified codon changes.