Figure 2.
Background-corrected fluorescence spectra of different concentrations of a mixture of QD-DiMan with Atto-594 labeled lectins for (A) a 1:1 ratio of QD:DC-SIGN, (B) a 1:10 ratio of QD:DC-SIGNR, and (C) a 1:1 ratio of QD:DC-SIGN-C. The corresponding apparent FRET ratio–protein concentration relationships at three different temperatures, fitted by eq 1, for (D) 1:1 mixed QD and DC-SIGN, (E) 1:10 mixed QD and DC-SIGNR, and (F) 1:1 mixed QD and DC-SIGN-C. Error bars represent the standard deviations (SDs) of triplicate experiments at each concentration.
