Table 3. Summary Kinetic Parameters for QD-DiMan Binding with DC-SIGN, DC-SIGN-C, and DC-SIGNR (SDs Represent Fitting Errors).
association
rate |
dissociation
rate |
||||||||
---|---|---|---|---|---|---|---|---|---|
lectin | ymax | kon /×107 M–1 s–1 | T1/2/s | a/×10–3 s–1 | y0 | yeq | koff/s–1 | t1/2/s | a/×10–3 s–1 |
DC-SIGN | 2.49 ± 0.03 | 2.24 ± 0.06 | 1.55 ± 0.04 | 10.3 ± 0.5 | 1.261 ± 0.007 | 0.129 ± 0.001 | 3.23 ± 0.03 | 0.215 ± 0.002 | 1.57 ± 0.09 |
DC-SIGN-C | 2.59 ± 0.01 | 2.92 ± 0.04 | 1.19 ± 0.02 | 5.1 ± 0.2 | 1.722 ± 0.006 | 0.131 ± 0.001 | 2.95 ± 0.02 | 0.235 ± 0.002 | 0.93 ± 0.05 |
DC-SIGNR | 0.0532 ± 0.0004 | 16 ± 1 | 0.22 ± 0.01 | –2.5 ± 0.2 | 0.067 ± 0.003 | 0.042 ± 0.001 | 0.45 ± 0.08 | 1.5 ± 0.3 | 0.02 ± 0.01 |