Table 2. Summary of the Cell Adhesion Results for the Studied Surfaces.
| Sample | Estimated bulk average surface RGD density (RGD units/nm2)a | Estimated maximum local average surface RGD density E (RGD units/nm2)b | Median cell density (cells/mm2)c | Median total FA area/cell (μm2)d |
|---|---|---|---|---|
| SAM PEG 100% | 0 | 0 | 6 | - |
| SAM RGD 100% | 1.22 | 1.22 | 115 | 66 |
| SAM RGD 10% | 0.122 | 0.122 | 81 | 18 |
| SAM RGD 1% | 0.0122 | 0.0122 | 17 | 12 |
| SAM BLANK-QS 100% | 0 | 0 | ||
| SAM RGD-QS 100% | 0.078 | 0.078 | 80 | 62 |
| SAM RGD-QS 10% | ∼0.0078–0.078 | 0.078 | 110 | 111 |
| SAM RGD-QS 1.5% | ∼0.0012–0.078 | 0.078 | 147 | 105 |
| SAM RGD-QS 0.125% | ∼0.0001–0.078 | 0.078 | 67 | 57 |
a
Estimated by calculating the packing density of the RGD units on a flat surface.
b
In mixed SAMs, RGD is not localized over QS nanovesicles, and therefore local and bulk density are the same. In hybrid SAMs, the local density of RGD units is the same as the density of RGD units on the QS surface and does not change with QS dilution on the SAM.