Table 2. Batch Synthesis of γ-E-Derivatives Catalyzed by BsGGT-GLX-AG.
| γ-E-derivative | reaction time (h) | conversion (%)a | product (mg)b | purity (%) | yield (%)c |
|---|---|---|---|---|---|
| γ-E-Met (1) | 3 | 30 | 117 | >99d | 28 |
| γ-E-(S)-allyl-Cys (2) | 3 | 27 | 100 | 88d | 20 |
| γ-E-Tau (3) | 24 | 44 | 188 | 90e | 40 |
| Ophthalmic acid (4) | 3 | 20 | 97 | 84d | 19 |
a
Determined by HPLC using calibration curves of the product.
b
After lyophilization of fractions collected from ion exchange column chromatography.
c
Referred to the pure product.
d
On the molar basis, estimated by NMR; the main impurity was γ-E-Gln which partially co-eluted with the product during ion-exchange column chromatography.
e
Estimated by HPLC.