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. 2022 Jul 7;122(20):15865–15913. doi: 10.1021/acs.chemrev.1c01031

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© 2022 The Authors. Published by American Chemical Society

Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).

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Automation of released N-glycan analysis by MALDI-TOF-MS. A graphical representation of the setup is provided and the consecutive processing steps are as follows: (1) PNGase F release, (2) ethyl esterification, (3) glycan enrichment by hydrophilic polypropylene (GH Polypro, GHP) HILIC–SPE, (4) glycans are eluted from the GHP membrane, and (5) MALDI target spotting followed by (6) MALDI-TOF–MS analysis. Reprinted with permission from ref (193). Copyright 2015 American Chemical Society.