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. 2022 Jul 7;122(20):15865–15913. doi: 10.1021/acs.chemrev.1c01031

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© 2022 The Authors. Published by American Chemical Society

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RP-LC-MS separation of tryptic Fc glycopeptides of a polyclonal serum IgG standard. (A) Extracted ion chromatograms (EIC) of the most abundant Fc glycopeptides per IgG subclass from polyclonal human serum IgG. The separation is mainly driven by the hydrophobicity of the peptide backbone and separate clusters are obtained where all glycoforms of a specific subclass elute. (B) Illustration of a summed mass spectrum of the cluster containing IgG Fc glycopeptides from subclass IgG1. Only the mass range of the triply charged species is displayed. IgG1 = EEQYNSTYR; IgG2/3 = EEQFNSTFR; IgG4 = EEQFNSTYR.